Blood cultures are taken to investigate the presence of bacteria in a sample of blood. Once you have watched this video, why not attempt the multiple choice quiz to test your knowledge?<\/p>\r\n","protected":false},"excerpt":{"rendered":"
Blood cultures are taken to investigate the presence of bacteria in a sample of blood. Once you have watched this video, why not attempt the multiple choice quiz to test your knowledge?<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-12","post","type-post","status-publish","format-standard","hentry","category-tomorrows-clinicians","entry"],"youtubeVideo":"481204919\/036dc8d604","question1":"Which of the following is an indication to take peripheral blood cultures?","answers1":"Dysuria| Fever| Productive cough| Headache and neck stiffness","correctAnswer1":"1","correctAnswerText1":"Persistent or repeated intermittent fever >37\u02daC or any fever >38\u02daC, with a clinical picture of likely infection should be investigated for a bacteraemia.","question2":"What steps should be performed in preparation for taking peripheral blood cultures?","answers2":"Clean tray with detergent wipe. Wash your hands.| Clean tray with alcohol based cleansing wipe. Disinfect your hands.| Clean tray with detergent then alcohol based cleansing wipes. Wash your hands.| Clean tray with detergent then alcohol based cleansing wipes. Disinfect your hands.\r\n","correctAnswer2":"2","correctAnswerText2":"The detergent cleans the tray. The alcohol based cleansing wipe then disinfects it. Washing rather than disinfecting your hands reduces the risk of spore-forming bacteria such as clostridium difficile. This is important to help maintain asepsis during the procedure.","question3":"What else should be disinfected?","answers3":"Skin| Top of culture bottles| Tourniquet\r\n| Skin and top of culture bottles\r\n","correctAnswerText3":"Both the patient's skin and the tops of the bottles should be disinfected. This is to reduce the risk of contaminating the blood culture sample from bacteria on the skin or bottle tops, and therefore reducing the rate of false positive results.","correctAnswer3":"3","question4":"What must you not do after cleaning the skin?","answers4":"Apply a tourniquet| Palpate the vein| Insert the needle| Discard the skin cleanser applicator\r\n","correctAnswer4":"1","correctAnswerText4":"Although sterile gloves are worn, re-palpating the vein after cleaning the skin increases the risk of contamination to the blood culture sample and is not regarded as acceptable aseptic technique.","question5":"How far from the site of needle insertion should the tourniquet be applied?","answers5":"2 cm| 5 cm| 10 cm| 20 cm\r\n","correctAnswer5":"2","correctAnswerText5":"The tourniquet should be placed approximately 10 cm away (or a hands width) from the insertion site. This is to avoid contaminating the sterile field.","question6":"What device(s) should be used to take peripheral blood cultures?","answers6":"Green needle and syringe| Butterfly needle and syringe| Blue needle and syringe| Blood collection set and holder","correctAnswer6":"3","correctAnswerText6":"A blood collection set with holder is the most suitable device for collecting a blood culture sample. The equipment is designed to minimise the risk of bacterial contamination and sharps injury.","question7":"Which blood culture bottles are typically used to take peripheral blood cultures?","answers7":"Aerobic and anaerobic| Anaerobic and fungal\r\n| Aerobic and fungal| Anaerobic and protazoal\r\n","correctAnswer7":"0","correctAnswerText7":"Aerobic and anaerobic culture bloods are used to culture aerobic and anaerobic bacteria.","question8":"Which culture bottle should be filled first?","answers8":"Fungal| Anaerobic| Protazoal| Aerobic\r\n","correctAnswer8":"3","correctAnswerText8":"The aerobic sample must be collected first. This is to ensure that any residual air in the blood collection set does not enter the anaerobic sample and affect the growth of any potential anaerobic culture, which would yield a false negative result.","question9":"How much blood should be collected in each culture bottle?","answers9":"5 ml| 10 ml| 15 ml| 20 ml\r\n","correctAnswer9":"1","correctAnswerText9":"Due to the relatively low yield of bacteria from blood, a 10 ml sample per culture is required to reduce the rate of false negative results.","question10":"For peripheral blood cultures, what information should be documented on the laboratory blood form?","answers10":"Date and patient details on microbiology form| Patient details and ward on a standard blood form| Patient details and date on a standard blood form| Ward and patient details on a microbiology form","correctAnswer10":"0","correctAnswerText10":"A microbiology form for blood cultures must include three key points of patient identification - full name, date of birth and patient identification number. The date of sample collection must also be recorded. The time of sample collection should also be documented, particularly if multiple samples are sent to the microbiology lab. Additional information such as the clinical details and any current antibiotic therapy may also be required.","question11":"","answers11":"","correctAnswer11":"","correctAnswerText11":"","question12":"","answers12":"","correctAnswer12":"","correctAnswerText12":"","question13":"","answers13":"","correctAnswer13":"","correctAnswerText13":"","question14":"","answers14":"","correctAnswer14":"","correctAnswerText14":"","question15":"","answers15":"","correctAnswer15":"","correctAnswerText15":"","icon":"taking-blood-cultures","parentId":"","recapId":"441","_links":{"self":[{"href":"https:\/\/vapp.rguc.co.uk\/index.php?rest_route=\/wp\/v2\/posts\/12"}],"collection":[{"href":"https:\/\/vapp.rguc.co.uk\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vapp.rguc.co.uk\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vapp.rguc.co.uk\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/vapp.rguc.co.uk\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=12"}],"version-history":[{"count":11,"href":"https:\/\/vapp.rguc.co.uk\/index.php?rest_route=\/wp\/v2\/posts\/12\/revisions"}],"predecessor-version":[{"id":782,"href":"https:\/\/vapp.rguc.co.uk\/index.php?rest_route=\/wp\/v2\/posts\/12\/revisions\/782"}],"wp:attachment":[{"href":"https:\/\/vapp.rguc.co.uk\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=12"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vapp.rguc.co.uk\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=12"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vapp.rguc.co.uk\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=12"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}